Some practical experience has been gained in chromatographic analysis, and I'd like to share it with everyone. 1. When it comes to reversed-phase chromatography, which is better between acetonitrile and methanol? Methanol and acetonitrile are the two most commonly used organic solvents in this type of analysis. While their polarities and solvent strengths are quite similar, there are still some key differences that can influence the separation efficiency and selectivity. For instance, acetonitrile generally offers a slightly higher elution strength compared to methanol, which means it can move compounds through the column more quickly. However, methanol tends to be less toxic and more cost-effective, making it a popular choice in many laboratories. Additionally, the choice between the two often depends on the nature of the analytes and the specific requirements of the method. Understanding these subtle differences can help improve the overall performance of your chromatographic system. It's always a good idea to test both solvents if possible, especially during method development.
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