Shanghai Xinzhuang Instrument Co., Ltd. is a leading company in the field of scientific instruments, committed to technological innovation and product development. With a focus on R&D, manufacturing, and sales, the company has made significant contributions to the advancement of molecular biology and life sciences. Their range of PCR machines and related equipment are widely used in research labs, diagnostic centers, and biotechnology companies around the world.
Understanding the use of PCR is essential for scientists working with DNA amplification. In eukaryotic organisms, the genome consists of both coding (exons) and non-coding (introns) regions. Because of this, directly using genomic DNA for gene expression studies can lead to issues, as the presence of introns may prevent proper protein synthesis. To overcome this challenge, researchers often turn to RNA extraction followed by reverse transcription PCR (RT-PCR), which allows for the isolation of mRNA and subsequent cDNA synthesis.
RNA extraction is a critical step in molecular biology workflows. The process involves breaking down cells or tissues using denaturants like guanidinium isothiocyanate, followed by separation using organic solvents such as phenol and chloroform. Careful handling is required, as even small amounts of RNase can degrade RNA, making it unsuitable for downstream applications. Special attention must be given to maintaining RNase-free conditions throughout the procedure.
To ensure high-quality RNA, several steps are crucial: using appropriate inhibitors like DEPC, avoiding contamination from both external and internal sources, and following standardized protocols. For instance, TRIZOL reagent provides an efficient method for isolating total RNA directly from cells or tissues, preserving RNA integrity during lysis.
Once RNA is extracted, RT-PCR is used to amplify specific sequences. This technique combines reverse transcription with PCR, enabling sensitive detection and quantification of gene expression. Proper primer design is key to successful RT-PCR. Primers should be 18–24 nucleotides long, have balanced GC content, avoid secondary structures, and minimize the risk of primer dimers.
The annealing temperature is another important factor in PCR. It should be set just below the Tm of the primers to ensure specificity while allowing efficient amplification. Adjusting the temperature gradually can help improve the accuracy of the results.
Shanghai Xinzhuang Instrument Co., Ltd. continues to innovate and provide reliable tools that support these complex procedures. Whether you're conducting basic research or advanced diagnostics, their PCR machines and related products are designed to meet the highest standards of performance and reliability. If you have any questions or need further assistance, feel free to reach out.These home decorations are the prevalent trends in modern interior design, can create a modern artistic atmosphere, make your room modern and style look, also its colors give a refreshing feel, adding color and life to the whole room.
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