Ultrasound to break down the activity of lactase

The milk contains about 4.6% of lactose. In order to be effectively absorbed in the intestine, lactose needs to be hydrolyzed to glucose and galactose by the action of lactase. Almost all infants have high lactase activity in the intestines. However, most people begin to reduce lactase activity after 3-5 years of age, which is about 90% to 95% lower. It is currently believed that the reduction of lactase activity in the intestine is controlled by a recessive gene and is a normal pattern of human physiological activity. Experiments have confirmed that lactase activity cannot be increased even by external stimuli such as continuous promotion of lactose absorption.

This process of decreasing lactase activity is known as the Lactase Nonpersistence (LNP) phenomenon. Lactose deficiency in the intestine of adults causes lactose to accumulate in the large intestine and become a fermentation substrate for intestinal microbes. As a result, it causes diarrhea, abdominal swelling, abdominal pain and flatulence. This phenomenon is called lactose intolerance. Lactose Intolerance.

There is a considerable proportion of people in the world, and Asians do not suffer from lactose intolerance, so lactose intolerance is getting more and more attention. Lactobacillus acidophilus has the ability to produce certain β-galactosidase, but since β-galactosidase is an intracellular enzyme, its detection requires cell wall breaking, and there are many methods for cell wall breaking, such as physical grinding, Chemical dissolution method, etc., but the effect is not very ideal, the vitality can not be accurately determined, so this experiment uses ultrasonic shredding method to break the cell of Lactobacillus acidophilus, trying to effectively detect the activity of β-galactosidase, which is acidophilic Lactobacillus is used as a functional strain and a probiotic factor in dairy products to provide a theoretical basis for effectively alleviating lactose intolerance.

Due to the different strains, the conditions for breaking the wall of the ultrasonic cells are different, and the vigor of the produced β-galactosidase is also different. Ultrasonic waves have a strong biological effect. When ultrasonic treatment is performed, the high-frequency vibration of the ultrasonic waves is inconsistent with the vibration of the microbial cells, causing a partial vacuum in the surrounding environment of the cells, causing the cell membrane to generate cavitation and thereby break it.

The efficiency of ultrasonic disruption depends on the audio frequency, sound energy, processing time, cell concentration and cell type. The use of ultrasound must be noted that the control intensity is within a certain limit, that is, just below the level at which the solution produces foam, because the foam will cause protein. Denaturation, too low strength will reduce the crushing efficiency. The test proves that foam will be generated when the power is greater than 240W. Therefore, the power selection is 200W and the maximum is not more than 220W. The value is slightly adjusted in the vicinity.

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