CAS: 9068-38-6, AMV Reverse Transcriptase, AMV Reverse Transcriptase Details jonln Service Commitment: To assure you that the products you sell are genuine, and issue formal ordinary or VAT invoices. With the invoice and purchase of goods, you can enjoy product guarantee services such as return, replacement, repair, etc. For details, please call CAS: 9068-38-6, AMV Reverse Transcriptase, AMV Reverse Transcriptase, AMV Reverse transcriptase English name: AMV Reverse transcriptase; Revertase; RNA-directed DNA polymerase other names: AMV reverse transcriptase CAS number: 9068-38-6 grade: BR source: purified Avian Myeloblastosis Virus (Avian Myeloblastosis Virus) particle concentration: 10u / ul Definition of activity: One unit is defined as: the amount of enzyme required to catalyze the incorporation of 1 nmol dTTP (deoxynucleotide) into an acid-insoluble substance within 10 minutes at 37 ° C. The reaction conditions were: 50mM Tris-HCl (pH 8.3, 25 ° C), 8.75mM MgCl2, 10mM DTT, 40mM KCl, 10mM DTT, 0.1mg / ml BSA, 1mM radiolabeled dTTP, 0.25mM poly (A) 400, 0.25mM oligo (dT) 50 quality control test: activity, SDS-PAGE / purity, DNase, RNase, endonuclease, RT-PCR, cDNA first strand synthesis Product description: AMV reverse transcriptase catalyzed by DNA, RNA or RNA: DNA polymerization with DNA hybrids as templates (1). It requires primers (DNA primers are more efficient than RNA primers) and Mg2 + or Mn2 +. The enzyme has inherent RNase H activity. Non-ionic surfactants and hydrogen sulfide compounds can increase the activity of the enzyme in vitro. Features: 1. High concentration product: Cat. # M9004 contains AMV RT with a concentration of 20-25u / ml 600 units. 2. Provide 5 × reaction buffer: 250mM Tris-HCl (pH 8.3, 25 ° C); 250mM KCl; 50mM MgCl2; 2.5mM spermine; 50mM DTT. 3. Temperature stability: Since AMV RT is more stable at higher reaction temperature (37-58 ℃), it is especially suitable for reverse transcription storage buffer with complex secondary structure template: 200mM potassium phosphate buffer (pH 7.2, 4 ° C), 0.2% Triton X-100, 2mM DTT and 50% (v / v) glycerol Usage: Synthesis of the first and second strand of cDNA. Primer extension and RNA sequencing (2). For RT-PCR, 10 ml of the product containing AMV RT and reaction buffer can be directly added to a 50 ml PCR reaction system containing Taq DNA polymerase. If you use GoTaq DNA Polymerase or PCR Master Mix, you can add up to 25ml of RT reaction product per 50ml of PCR reaction system: suspension, α β-type whole enzyme with molecular weight of about 157000 Daltons separated from AMV. It has ribonuclease H activity itself: biochemical research. Under the action of AMV reverse transcriptase, the process of reverse transcription from RNA to cDNA can be completed, and then the cDNA can be amplified by optimized U-Taq DNA polymerase. Storage: -20 ℃
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