How to safely use the biological safety cabinet
Shanghai Xinzhuang Instrument Co., Ltd. Xinzhuang (LNB) brand biosafety cabinet is used to protect the operator and the laboratory environment when it is used to process infectious materials such as primary cultures, bacterial strains and diagnostic specimens. The materials are designed to avoid exposure to infectious aerosols and spills that may occur during the above operations.
Shanghai Xinzhuang Instruments teaches you how to use biosafety cabinets safely:
1. The speed at which the position air enters the biosafety cabinet through the front opening is approximately 0.45 m/s. Directional airflow at such speeds is highly susceptible to interference, including airflow from personnel approaching the biosafety cabinet, opening windows, air supply system adjustments, and opening and closing doors.
Therefore, it is desirable that the biosafety cabinet be located in a place where personnel activities, items flow, and where airflow may be disturbed. Keep as much as 30 cm of space behind the safety cabinet and on each side to take advantage of the maintenance of the safety cabinet. A space of 30-35 cm should be left on the top of the safety cabinet to accurately measure the speed of the air passing through the exhaust filter and to facilitate the replacement of the exhaust filter.
2. If the operator's biosafety cabinet is used improperly, its protective effect may be greatly affected. Operators need to be careful to maintain the integrity of the airflow in the front opening as they move in and out of the safety cabinet. The arms should move vertically in and out of the front opening.
The hand and arms are extended into the biosafety cabinet for about one minute, so that the safety cabinet can be adjusted and the air inside can be swept over the surface of the hands and arms before the item can be processed. Place all necessary items in the safety cabinet before starting the experiment to minimize the number of times your arms enter and exit the front opening.
3. Item placement The air intake grille on the front of the secondary biosafety cabinet cannot be blocked by paper, equipment or other items. Items placed in the safety cabinet should use 70% alcohol to eliminate surface contamination. Experiments can be performed on a towel soaked with disinfectant to absorb droplets that may be spilled. All items should be placed as close as possible to the edge of the workbench at the back of the workbench and will not block the alternate grille during operation. Equipment that produces aerosols (such as mixers, centrifuges, etc.) should be placed close to the rear of the cabinet. Large-volume items such as biohazard waste bags, trays containing discarded straws, and suction filters should be placed on one side of the cabinet. The experimental operation on the work surface should be in the direction from the clean area to the contaminated area.
The autoclavable biohazard waste bags and the suction cups should not be placed outside the safety cabinet. Otherwise, the arms must frequently enter and exit the safety cabinet when using these items, which will interfere with the integrity of the air barrier of the safety cabinet. This affects the protection of people and items.
4. Operation and Maintenance Most biosafety cabinets are designed to work 24 hours a day. The researchers also found that continuous work helps control the levels of dust and particles in the laboratory. The Class A1 and Type A2 biosafety cabinets that are vented to the room or connected to a dedicated exhaust duct through the casing interface can be closed when not in use. Other biosafety cabinets, such as secondary B1 and secondary B2, are installed through rigid tubes and must always maintain air flow to maintain room air balance. Before starting work and after completing work, the safety cabinet should be operated for at least 5 minutes to complete the “purification†process, which should be left in the time when the polluted air is sent out of the safety cabinet.
5. UV lamps are not required in the UV lamp biosafety cabinet. If UV lamps are used, they should be cleaned weekly to remove dust and dirt that may affect their bactericidal effect. When re-certifying the cabinet, check the intensity of the UV light to ensure proper light emission. Always turn off the UV lamp when someone is in the room to protect your eyes and skin from inadvertent exposure.
6. Open flames should be avoided in an environment where there is almost no microorganisms formed in the biosafety cabinet. The use of open flames can have an effect on the airflow and can be dangerous when dealing with volatile and flammable materials. When sterilizing the inoculating loop, a micro-burner or an electric furnace can be used instead of an open flame.
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