Bacteria is directly microscopically inspected without staining, and is mainly used to check the power and movement of bacteria in the state of life.
Commonly used methods are the pressure drop method and the hanging drop method, which are observed with an ordinary optical microscope. Observed by dark field microscope or phase contrast microscope, the effect is better. If the bacteria are motivated, you can see the bacteria move from one place to another with obvious directional displacement; if the bacteria are not motivated, the water molecules hit the bacteria to show Brownian motion, and only vibrate in place without change in position.
The capillary method is used to check the power of anaerobic bacteria.
In addition to bacterial specimens, spirochetes are not easily stained and have morphological characteristics, so they are often used for dark-field microscopy.
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